Phytochemical Profile of Urtica dioica L. by Gas Chromatography-Mass Spectrometry GC-MS and Antioxidant Activity

Authors

  • Aseel K. Alanbari University of Diyala
  • Ragha Safaa Alddin University of Diyala
  • Wurood Khald Mohammed University of Diyala

DOI:

https://doi.org/10.21070/nabatia.v13i1.1649

Keywords:

Stinging Nettle, Polyphenolic Compounds, Chemical Profile, Biological Activity

Abstract

Leaves are rich in many phytochemical compounds, all bioactive compounds formed normally during plants metabolism as  secondary  products. The  phytochemical compounds  screened by gas chromatography-mass spectrometry (GC-MS) method. Twenty nine  bioactive phytochemical compounds  were  identified  in  the  .  The  GC-MS  analysis  of  U. dioica  revealed  the  identification  of phytochemical  compounds  is  based  on  the  peak  area,  retention  time  ., as well as the antioxidant activities of the leaves were determine. . the first compound was benzene that presence at 5.339 min with peak  area 1.18, while the 9-Octadecenamide was the last compound shown at 68.304 min with 2.18 peak area. Including  organic compounds, fatty acids and phenols.  A DPPH radical scavenging e results showed U. dioica extract at 600 concentrations high than ascorbic acid was93.76 µg/ml while aceorbic acid was 91.14 µg/ml. this finding indicate that U. dioica have high antioxidant ability.  . This  manuscript to cover  the  chemical  composition  of  methanolic  extract  of  U.  dioica leaves  ( stinging nettile)  that growing naturally in Iraq, as a chemical study and provide details of pharmacological propos

References

G. Kavalali, H. Tuncel, S. Göksel, and H. H. Hatemi, “Hypoglycemic activity of Urtica pilulifera in streptozotocin-diabetic rats,” J. Ethnopharmacol., vol. 84, no. 2, pp. 241–245, 2003, doi: https://doi.org/10.1016/S0378-8741(02)00315-X.

D. Kregiel, E. Pawlikowska, and H. Antolak, “Urtica spp.: Ordinary plants with extraordinary properties,” Molecules, vol. 23, no. 7, 2018, doi: 10.3390/molecules23071664.

L. Grauso, B. de Falco, V. Lanzotti, and R. Motti, “Stinging nettle, Urtica dioica L.: botanical, phytochemical and pharmacological overview,” Phytochem. Rev., vol. 19, no. 6, pp. 1341–1377, 2020, doi: 10.1007/s11101-020-09680-x.

S. Durovic et al., “Chemical Constituents of Stinging Nettle (Urtica dioica L.): A Comprehensive Review on Phenolic and Polyphenolic Compounds and Their Bioactivity,” Int. J. Mol. Sci., vol. 25, no. 6, 2024, doi: 10.3390/ijms25063430.

J. Mutke, R. Jacobs, K. Meyer, T. Henning, and M. Weigend, “Diversity patterns of selected Andean plant groups correspond to topography and habitat dynamics, not orogeny,” Front. Genet., vol. 5, no. SEP, 2014, doi: 10.3389/fgene.2014.00351.

A. Kumar, A. Singh Bisht, S. Joshi, D. Juyal, and C. Arun Kumar, “Pharmacognostical and phytochemical study of a plant Urtica parviflora Roxb.-A review,” ~ 42 ~ J. Pharmacogn. Phytochem., vol. 6, no. 3, pp. 42–45, 2017.

H. P. Devkota et al., “Stinging Nettle (Urtica dioica L.): Nutritional Composition, Bioactive Compounds, and Food Functional Properties,” Molecules, vol. 27, no. 16, 2022, doi: 10.3390/molecules27165219.

A. Uysal et al., “Chemical and biological insights on Cotoneaster integerrimus: A new (-)- epicatechin source for food and medicinal applications,” Phytomedicine, vol. 23, no. 10, pp. 979–988, 2016, doi: https://doi.org/10.1016/j.phymed.2016.06.011.

A. Güder and H. Korkmaz, “Evaluation of in-vitro Antioxidant Properties of Hydroalcoholic Solution Extracts Urtica dioica L., Malva neglecta Wallr. and Their Mixture.,” Iran. J. Pharm. Res. IJPR, vol. 11, no. 3, pp. 913–923, 2012.

D. Komes, A. B. Ak-Cvitanovi, D. Hori, G. Rusak, S. Liki, and M. Berendika, “Phenolic composition and antioxidant properties of some traditionally used medicinal plants affected by the extraction time and hydrolysis.,” Phytochem Anal, vol. 22, pp. 172–180, 2011.

G. Zengin et al., “Euphorbia denticulata Lam.: A promising source of phyto-pharmaceuticals for the development of novel functional formulations,” Biomed. Pharmacother., vol. 87, pp. 27–36, 2017, doi: https://doi.org/10.1016/j.biopha.2016.12.063.

S. A. Dar, F. A. Ganai, A. R. Yousuf, M.-H. Balkhi, T. M. Bhat, and P. Sharma, “Pharmacological and toxicological evaluation of Urtica dioica,” Pharm. Biol., vol. 51, no. 2, pp. 170–180, 2013, doi: 10.3109/13880209.2012.715172.

A. N. M. Alamgir, A. Rahman, and M. Rahman, “Secondary Metabolites and Antioxidant Activity of the Crude Leaf Extract of Bacopa monniera (L.) Pennel. and Coccinia grandis (L.) J. Voigt,” ~ 226 ~ J. Pharmacogn. Phytochem., vol. 3, no. 1, pp. 226–230, 2014.

S. Đurović et al., “Chemical composition of stinging nettle leaves obtained by different analytical approaches,” J. Funct. Foods, vol. 32, pp. 18–26, 2017, doi: https://doi.org/10.1016/j.jff.2017.02.019.

Z. Z. Kukrić et al., “Characterization of antioxidant and antimicrobial activities of nettle leaves (Urtica dioica L.),” Acta Period. Technol., vol. 43, pp. 257–272, 2012, doi: 10.2298/APT1243257K.

D. Orčić et al., “Quantitative determination of plant phenolics in Urtica dioica extracts by high-performance liquid chromatography coupled with tandem mass spectrometric detection,” Food Chem., vol. 143, pp. 48–53, 2014, doi: https://doi.org/10.1016/j.foodchem.2013.07.097.

X. Luo et al., “Therapeutic effects of total coumarins from Urtica dentata Hand on collagen-induced arthritis in Balb/c mice,” J. Ethnopharmacol., vol. 138, no. 2, pp. 523–529, 2011, doi: https://doi.org/10.1016/j.jep.2011.09.050.

M. Ş. Erbay, S. Anıl, and G. Melikoğlu, “Plants Used as Painkiller in Folk Medicine in Turkey-I STOMACHACHE,” Marmara Pharm. J., vol. 21, no. 4, pp. 741–755, 2017.

S. Sitrallah and J. Merza, “hemical composition of essential oil extracted from urtica pilulifera and evaluation its biological activity,” Food Sci. Qual. Manag., vol. 78, no. 44–48, 2018.

B. C. Joshi and S. Uniyal, “Establishment of quality control protocols and antioxidant activity of Urtica dioica L.,” ournal Conv. Knowl. Holist. Heal., vol. 1, no. 1, pp. 1–6, 2017.

G. A. Omar and L. Y. Mohammed, “PHYSIOCHEMICAL STANDARDIZATION AND PHYTOCHEMICAL SCREENING OF Urtica dioica L. LEAVES GROWING IN ZAKHO, KURDISTAN REGION, IRAQ,” Sci. J. Univ. Zakho, vol. 11, no. 3, pp. 306–316, 2023, doi: 10.25271/sjuoz.2023.11.3.1069.

S. Durovic et al., “Recovery of Polyphenolic Compounds and Vitamins from the Stinging Nettle Leaves: Thermal and Behavior and Biological Activity of Obtained Extracts,” Molecules, vol. 28, no. 5, 2023, doi: 10.3390/molecules28052278.

T. Özen and H. Korkmaz, “Modulatory effect of Urtica dioica L. (Urticaceae) leaf extract on biotransformation enzyme systems, antioxidant enzymes, lactate dehydrogenase and lipid peroxidation in mice,” Phytomedicine, vol. 10, no. 5, pp. 405–415, 2003, doi: https://doi.org/10.1078/0944-7113-00275.

Downloads

Published

2025-02-23

How to Cite

Alanbari, A. K., Alddin, R. S., & Mohammed, W. K. . (2025). Phytochemical Profile of Urtica dioica L. by Gas Chromatography-Mass Spectrometry GC-MS and Antioxidant Activity. Nabatia, 13(1), 19–40. https://doi.org/10.21070/nabatia.v13i1.1649