Evaluation of the Biological Activity of some Plant Extracts Against Gram-Positive and Gram-negative Bacteria

Authors

  • Mohammad Kareem Hbaiel Al-Shatrah University
  • Mohammed Ali Kassab Al-Shatrah University
  • Abdullah Hijaz Hashim Al-Shatrah University

DOI:

https://doi.org/10.21070/nabatia.v13i2.1657

Keywords:

Biological activity, plant extracts, P. aeruginosa, B. cereus

Abstract

The inhibitory activity of two types of plant extracts, namely the alcoholic and aqueous extracts of Capparis and Artemisia, on the growth of two types of biologically important bacteria included Bacillus cereus, a Gram-positive bacterium, and Pseudomonas aeruginosa, a Gram-negative bacterium, one of the most important opportunistic pathogens.The plant extracts were prepared at different concentrations (10, 20, and 30 mg/ml), and their effect was tested using the diffusion method in the Mueller-Hinton medium. The results showed that the alcoholic extracts were more effective in inhibiting the growth of both bacteria types than the aqueous extracts. The study demonstrated that plant extracts containing active chemical compounds, particularly alcoholic ones, could be used as effective natural antibiotics in healthcare. To pave the way for further comprehensive studies to identify these compounds, their effects, and their potential benefits. These results may contribute to the development of natural therapeutic alternatives to combat bacterial infections or be used to indicate the effectiveness of active chemical compounds present in plants by inhibiting B.  cereus.

References

[1] U. Owhe-Ureghe, D. Ehwarieme, and D. Eboh, “Antibacterial activity of garlic and lime on isolates of extracted carious teeth,” 2010. https://www.ajol.info/index.php/ajb/article/view/80587

[2] I. A. Ameen and H. F. Okab, “Phyto-activity of Syzygium aromaticum extract against pathogenic bacteria isolated from chronic tonsilitis patients,” Romanian Journal of Infectious Diseases, vol. 27, no. 1, pp. 5–10, Mar. 2024, doi: 10.37897/rjid.2024.1.1.

[3] B. a. A. Sattar, A. M. Hassan, and A. S. Hassan, “In vitro antimicrobial activity of Thymus Vulgaris,Origanum vulgare and Rosmarinus officinalis against dental caries pathogens,” 2012. https://jih.uobaghdad.edu.iq/index.php/j/article/view/610

[4] R. A. AlAskary and H. R. Malih, “Effectiveness study of Artemisia herba-alba and Borage officinalis leaf extract against bacteria Staphylococcus aureus,” May 17, 2021. https://jsci.utq.edu.iq/index.php/main/article/view/787

[5] A. Nadeem, M. Mujeeb, M. Amir, and A. Waseem, “Impact of Biodiversity on Umbelliferone Content of Aegle marmelos (Rutaceae),” Planta Medica, vol. 76, no. 05, Mar. 2010, doi: 10.1055/s-0030-1251799.

[6] A. Sadiq, et al., “Ethnopharmacology of Artemisia annua L.: A review”, Artemisia annua-pharmacology and biotechnology, 2013, pp. 9-25.‏

[7] W.-S. Kim et al., “Anti-inflammatory, antioxidant and antimicrobial effects of artemisinin extracts fromArtemisia AnnuaL.,” Korean Journal of Physiology and Pharmacology, vol. 19, no. 1, p. 21, Jan. 2014, doi: 10.4196/kjpp.2015.19.1.21.

[8] M. P. Unesp Soares et al., “In vitro antibacterial activity of ethanol extract of Artemisia annua and its bioactive fractions against fish pathogens,” Apr. 01, 2021. https://repositorio.unesp.br/items/41c2f194-d1ff-44c8-959e-10df31cfd67e

[9] G. M, Adwan, G. I. Omar, “Evaluation of antimicrobial activity and genotoxic potential of Capparis spinosa (L.) plant extracts”, Microbiol. Res. J. Int, vol. 1, no. 21, Jan 2021, pp. 48-57.‏

[10] N. Abdel-Razek, N. El-Sabbagh, R. H. Khalil, and M. Abdel-Tawwab, “Prophylactic effects of dietary caper (Capparis spinosa) extracts on the control of Streptococcus agalactiae infection, growth, immune-antioxidant, and inflammation cytokine responses of Nile tilapia fingerlings,” Fish & Shellfish Immunology, vol. 142, p. 109126, Sep. 2023, doi: 10.1016/j.fsi.2023.109126.

[11] S. V. P. I. Abraham, A. Palani, B. R. Ramaswamy, K. P. Shunmugiah, and V. R. Arumugam, “Antiquorum Sensing and Antibiofilm Potential of Capparis spinosa,” Archives of Medical Research, vol. 42, no. 8, pp. 658–668, Nov. 2011, doi: 10.1016/j.arcmed.2011.12.002.

[12] C. Tong et al., “First insights into the prevalence, genetic characteristics, and pathogenicity of Bacillus cereus from generations worldwide,” mSphere, vol. 9, no. 11, Oct. 2024, doi: 10.1128/msphere.00702-24.

[13] W. G. Shahrour et al., “In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L.,” Notulae Botanicae Horti Agrobotanici Cluj-Napoca, vol. 52, no. 1, p. 13609, Mar. 2024, doi: 10.15835/nbha52113609.

[14] S. M, Ismail, G. M. Adwan, N. R. Jarrar, “Evaluation of Antimicrobial and Genotoxic Activity of Ephedra foeminea Ethanolic and Aqueous Extracts on Escherichia coli,” Mar. 2020. [Online]. Available: https://jjbs.hu.edu.jo/files/vol13/n1/Paper%20Number%2018.pdf

[15] S. Kasmiyati, E. Kristiani, and M. Herawati, “Antibacterial Activity and Secondary Metabolite Content of Polyploid Mutant of Artemisia cina Berg ex Poljakov,” Advances in Biological Sciences Research/Advances in Biological Sciences Research, Jan. 2021, doi: 10.2991/absr.k.210810.039.

[16] H. C. Kassab et al., “Antimicrobial Activity of Plant Extracts of Artemisia, Citrullus and Capparis”, South Eastern European Journal of Public Health. No. 2, 2024, pp. 45-51 https://doi.org/10.70135/seejph.vi.955

[17] T. Gull et al., (2015). “Antibacterial potential of Capparis spinosa and Capparis decidua extracts”, International journal of Agriculture and Biology, No. 4, vo. 17, 2015, pp. 727‒733.

[18] A. Sharma, S. Chandraker, V. Patel, and P. Ramteke, “Antibacterial activity of medicinal plants against pathogens causing complicated urinary tract infections,” Indian Journal of Pharmaceutical Sciences, vol. 71, no. 2, p. 136, Jan. 2009, doi: 10.4103/0250-474x.54279.

[19] S. Iqbal, et al., “Antioxidant properties of methanolic extracts from leaves of Rhazya stricta”, Journal of medicinal food, no. 2, vol 9, 2006, pp. 270-275.‏

[20] K. B, Arangale, S. S. Kalokhe, A. R. Tuwar, “Antimicrobial Activity and Phytochemical Analysis of Capparis grandis Linn,” International Journal of Scientific Research in Science and Technology, pp. 255–260, May 2022, doi: 10.32628/ijsrst218613.

[21] B. Sultana, F. Anwar, and M. Ashraf, “Effect of Extraction Solvent/Technique on the antioxidant activity of selected medicinal plant extracts,” Molecules, vol. 14, no. 6, pp. 2167–2180, Jun. 2009, doi: 10.3390/molecules14062167.

[22] A. Hussain, M. Sajid, H. Rasheed, M. Hassan, M. A. Khan, and S. A. I. Bokhari, “Phytochemistry and antibacterial efficacy of northeastern pakistani Artemisia rutifolia stephan ex spreng. extracts against some clinical and phyto-pathogenic bacterial strains,” ACTA Pharmaceutica Sciencia, vol. 60, no. 3, p. 247, Jan. 2022, doi: 10.23893/1307-2080.aps.6017.

[23] K. Chebbac et al., “Antimicrobial and Antioxidant Properties of Chemically Analyzed Essential Oil of Artemisia annua L. (Asteraceae) Native to Mediterranean Area,” Life, vol. 13, no. 3, p. 807, Mar. 2023, doi: 10.3390/life13030807.

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Published

2025-06-07

How to Cite

Hbaiel, M. K., Kassab, M. A., & Hashim, A. H. (2025). Evaluation of the Biological Activity of some Plant Extracts Against Gram-Positive and Gram-negative Bacteria. Nabatia, 13(2), 96–103. https://doi.org/10.21070/nabatia.v13i2.1657