Study of The Effect of Boswellia Leaf Extract on The Activity of Cardiac Enzymes (ALP, AST, ALT, LDH) Using Spectroscopic Methods
Abstract
This study investigates the impact of Boswellia carterii leaf extracts on serum enzyme activity in myocardial infarction (MI) patients. Employing a comparative analysis between 60 healthy individuals and 40 cardiac patients, we measured the serum activity of ALP, AST, ALT, and LDH enzymes. Our findings reveal elevated enzyme activity in MI patients. We explored the inhibitory effects of aqueous, alcoholic, and ethyl acetate extracts of Boswellia carterii on these enzymes, demonstrating non-competitive inhibition. The inhibition ratio of ALT, ALP, and AST post-treatment was quantitatively assessed. Statistical analysis was conducted using SPSS-26 with independent t-tests. This research highlights the potential therapeutic role of Boswellia carterii in modulating enzyme activity associated with cardiac events, suggesting avenues for novel treatments in cardiac care.
References
M. O. A. M. M. Al-shawk and H. Y. Ali, “Echocardiographic Estimation of Infarct Size By Using Cardiac Biomarkers (Troponin I,CK And CK-MB) And Some Hematological Changes in Patients With STEMI,” vol. 11, no. 3. pp. 528–538, 2014.
G. L. Koerbin, “High Sensitivity Troponin - Its Use in Diagnosis of Cardiac Dysfunction,” pp. 1–342, 2014, [Online]. Available: papers3://publication/uuid/309B358B-BA7D-46DC-8F16-3451074CDC8B%0Afile:///Users/tomkaier/Backup/Papers Library/Files/54/54227CF9-F40D-4E4D-BB22-BACCC25ABA50.pdf
S. Bhatia, “Introduction to enzymes and their applications,” Introd. to Pharm. Biotechnol. Vol. 2, pp. 1-1-1–29, 2018, doi: 10.1088/978-0-7503-1302-5ch1.
J. Li, Z. Zhao, H. Jiang, M. Jiang, G. Yu, and X. Li, “Predictive value of elevated alanine aminotransferase for in-hospital mortality in patients with acute myocardial infarction,” BMC Cardiovasc. Disord., vol. 21, no. 1, pp. 1–9, 2021, doi: 10.1186/s12872-021-01903-z.
M. Gao, Y. Cheng, Y. Zheng, W. Zhang, L. Wang, and L. Qin, “Association of serum transaminases with short- and long-term outcomes in patients with ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention,” BMC Cardiovasc. Disord., vol. 17, no. 1, pp. 1–8, 2017, doi: 10.1186/s12872-017-0485-6.
M. Cheraghi, H. Ahmadvand, A. Maleki, E. Babaeenezhad, S. Shakiba, and F. Hassanzadeh, “Oxidative Stress Status and Liver Markers in Coronary Heart Disease,” Reports Biochem. Mol. Biol., vol. 8, no. 1, pp. 49–55, 2019.
H. A. Hashim, A. K. Ibraheem, and S. S. Abbood, “Study of the Effect of Ginkgo Biloba Leaf Extracts on the Activity of Cardiac Enzymes LDH , CK-MB using Spectroscopic Methods CAJMNS,” no. c, pp. 891–902, 2023.
“8-Determination of Vitamins, Trace Elements, and Phytochemical Compounds in Boswellia carterii Leaves Extracts.”
I. P. Ezeugwunne, “Activities of Pancreatic Enzymes and Anthropometric Indices in Type 2 Diabetic Patients Attending Nnamdi Azikiwe University Teaching Hospital Nnewi, South Eastern, Nigeria,” Int. J. Curr. Sci. Res. Rev., vol. 04, no. 08, 2021, doi: 10.47191/ijcsrr/v4-i8-16.
C. Biolabs, “Aspartate Aminotransferase (AST) activity assay kit (colorimetric),” Aspartate Amin. Act. assay kit, pp. 1–8, 2019.
A. Names, N. M. Aziz, and A. History, “Effect of postmenopausal on some liver enzymes in Kirkuk women ’ s Authors Names,” no. 26, pp. 17–23, 2021.
N. A. Khadse, M. P. Deshmukh, and A. M. Wankhade, “Cardioprotective Activity Of Herbal Formulation In Experimental Animal,” Am. J. PharmTech Res., vol. 10, no. 5, pp. 15–27, 2020, doi: 10.46624/ajptr.2020.v10.i5.002.
D. S. Nirwan and U. Kumar, “International Journal of Medical Science and Education An official Publication of Association for Scientific and Medical Education ( ASME ) Original Research Article COMPARATIVE STUDY OF SERUM AST , CK-MB , IN MYOCARDIAL INFARCTION WITH SURVIVAL AND MYOCA,” vol. 5, no. June, pp. 182–187, 2018.
J. E. Adams, D. R. Abendschein, and A. S. Jaffe, “Biochemical markers of myocardial injury: Is MB creatine kinase the choice for the 1990s?,” Circulation, vol. 88, no. 2, pp. 750–763, 1993, doi: 10.1161/01.cir.88.2.750.
A. D. Anastasilakis et al., “Circulating irisin levels are lower in patients with either stable coronary artery disease (CAD) or myocardial infarction (MI) versus healthy controls, whereas follistatin and activin A levels are higher and can discriminate MI from CAD with similar to CK,” Metabolism., vol. 73, no. Mi, pp. 1–8, 2017, doi: 10.1016/j.metabol.2017.05.002.
M. Kosmopoulos et al., “The Emerging Role of Bone Markers in Diagnosis and Risk Stratification of Patients With Coronary Artery Disease,” Angiology, vol. 70, no. 8, pp. 690–700, 2019, doi: 10.1177/0003319718822625.
P. C. Oh et al., “Prognostic impact of alkaline phosphatase measured at time of presentation in patients undergoing primary percutaneous coronary intervention for ST-segment elevation myocardial infarction,” PLoS One, vol. 12, no. 2, pp. 1–11, 2017, doi: 10.1371/journal.pone.0171914.
S. K. Kunutsor, S. J. L. Bakker, J. E. Kootstra-Ros, R. T. Gansevoort, J. Gregson, and R. P. F. Dullaart, “Serum alkaline phosphatase and risk of incident cardiovascular disease: Interrelationship with high sensitivity C-reactive protein,” PLoS One, vol. 10, no. 7, pp. 1–16, 2015, doi: 10.1371/journal.pone.0132822.
A. A. Khan, K. S. Allemailem, F. A. Alhumaydhi, S. J. T. Gowder, and A. H. Rahmani, “The Biochemical and Clinical Perspectives of Lactate Dehydrogenase: An Enzyme of Active Metabolism,” Endocrine, Metab. Immune Disord. - Drug Targets, vol. 20, no. 6, pp. 855–868, 2019, doi: 10.2174/1871530320666191230141110.
A. Shamshirian et al., “Levels of Blood Biomarkers among Patients with Myocardial Infarction in Comparison to Control Group,” Ethiop. J. Health Sci., vol. 30, no. 1, pp. 5–12, 2020, doi: 10.4314/ejhs.v30i1.2.
M. W. HAMOLSKY and N. O. KAPLAN, “Measurements of enzymes in the diagnosis of acute myocardial infarction.,” Circulation, vol. 23, no. January, pp. 102–110, 1961, doi: 10.1161/01.CIR.23.1.102.
Y. Wu et al., “Serum lactate dehydrogenase activities as systems biomarkers for 48 types of human diseases,” Sci. Rep., vol. 11, no. 1, pp. 1–8, 2021, doi: 10.1038/s41598-021-92430-6.
O. A. Khalil, K. S. Ramadan, A. H. Hamza, and S. E. El-toukhy, “Association of plasma protein C levels and coronary artery disease in men,” vol. 12, no. 50, pp. 6986–6991, 2013, doi: 10.5897/AJB12.2013.
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